Journal: Materials Today Bio

Article Title: Activation of a passive, mesoporous silica nanoparticle layer through attachment of bacterially-derived carbon-quantum-dots for protection and functional enhancement of probiotics

doi: 10.1016/j.mtbio.2022.100293

Figure Lengend Snippet: Antibiotic treatment of E. coli colonized epithelial cell layers assisted by encapsulated B. infantis. Intestinal epithelial layers were colonized during 2 ​h by E . coli ATCC 25922 and subsequently co-cultured for 3 ​h in presence of tetracycline (10 ​μg ​mL −1 ) assisted by differently encapsulated B. infantis . (A) Number of E. coli CFUs adhering to intestinal epithelial layers after tetracycline treatment only or assisted by unencapsulated B. infantis , B. infantis encapsulated in a mesoporous silica nanoparticle layer in absence ( B. infantis @SiO 2 ) or presence of attached CQDs derived from L. acidophilus ATCC 4356 (L-CQDs) or E. coli ATCC 25922 (E-CQDs). (B) Same as panel A, now for the number of B. infantis CFUs adhering to intestinal epithelial layers. (C) Surface coverage of a transwell membrane by intestinal epithelial cell layers colonized by E. coli after B. infantis assisted tetracycline treatment. The surface coverage of a cell layer in absence of E. coli or B. infantis assisted tetracycline treatment was set at 100%. All error bars indicate standard deviations over triplicate experiments with separately cultured cells and bacteria. Statistically significant differences (p ​< ​0.05, Student t-test) are indicated by spanning bars with asterisks, while differences with respect to E. coli only, i.e. in absence of probiotic bacteria, are indicated by hash-decks.

Article Snippet: Human intestinal epithelial cells (Caco-2 BBe, ATCC CRL-2102) were prepared in a transwell model, as described before [ ].

Techniques: Cell Culture, Derivative Assay, Membrane, Bacteria